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35218 bla tem 1 genes  (ATCC)


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    ATCC 35218 bla tem 1 genes
    Effect of increasing tazobactam concentrations on E. coli susceptibility to TZP. MICs were determined for 907355 (A) and ATCC <t>35218</t> (B) via BMD in the presence of piperacillin (PIP) alone or TZP formulations containing different fixed concentrations (2 to 64 µg/ml) of tazobactam (TAZ). The MIC values in the legend are based on the concentration of piperacillin in the TZP formulation that inhibited growth. The range of MIC values obtained from six experiments is represented within each stacked bar on the graphs.
    35218 Bla Tem 1 Genes, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4780 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/35218+bla+tem+1+genes/pmc05915731-86-11-10?v=ATCC
    Average 99 stars, based on 4780 article reviews
    35218 bla tem 1 genes - by Bioz Stars, 2026-07
    99/100 stars

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    1) Product Images from "Extensive Gene Amplification as a Mechanism for Piperacillin-Tazobactam Resistance in Escherichia coli"

    Article Title: Extensive Gene Amplification as a Mechanism for Piperacillin-Tazobactam Resistance in Escherichia coli

    Journal: mBio

    doi: 10.1128/mBio.00583-18

    Effect of increasing tazobactam concentrations on E. coli susceptibility to TZP. MICs were determined for 907355 (A) and ATCC 35218 (B) via BMD in the presence of piperacillin (PIP) alone or TZP formulations containing different fixed concentrations (2 to 64 µg/ml) of tazobactam (TAZ). The MIC values in the legend are based on the concentration of piperacillin in the TZP formulation that inhibited growth. The range of MIC values obtained from six experiments is represented within each stacked bar on the graphs.
    Figure Legend Snippet: Effect of increasing tazobactam concentrations on E. coli susceptibility to TZP. MICs were determined for 907355 (A) and ATCC 35218 (B) via BMD in the presence of piperacillin (PIP) alone or TZP formulations containing different fixed concentrations (2 to 64 µg/ml) of tazobactam (TAZ). The MIC values in the legend are based on the concentration of piperacillin in the TZP formulation that inhibited growth. The range of MIC values obtained from six experiments is represented within each stacked bar on the graphs.

    Techniques Used: Concentration Assay, Formulation

    Effects of TZP on E. coli 907355 Bla TEM-1 levels and β-lactamase enzyme activity. (A) Detection of Bla TEM-1 in E. coli ATCC 25922 (lane 2), ATCC 35218 (lanes 3 to 5), and 907355 (lanes 6 to 9) by Western immunoblotting. Strains were cultured in medium containing no antibiotic (lanes 2, 3, and 6), 4 µg/ml tazobactam (lanes 4 and 7), 4 µg/ml piperacillin (lanes 5 and 8), or 4/4 µg/ml TZP (lane 9) prior to protein sample collection. Molecular mass standards are shown in lane 1 (kilodaltons are indicated to the left of the blot). The estimated molecular mass of Bla TEM-1 is 31.5 kDa. This experiment was repeated on independently collected samples and yielded similar results. (B) Determination of Bla TEM-1 activity in E. coli 907355 via a nitrocefin hydrolysis assay after growth in the presence or absence of 4/4 µg/ml TZP. ATCC 35218 Bla TEM-1 activity is included as a comparison but was not determined (ND) in the presence of TZP due to lack of growth. Units on the y axis denote the micromoles of nitrocefin hydrolyzed per milligram of total protein per minute. The values above each bar on the graph represent the means of 3 experiments, and the error bars indicate the standard deviations of the means.
    Figure Legend Snippet: Effects of TZP on E. coli 907355 Bla TEM-1 levels and β-lactamase enzyme activity. (A) Detection of Bla TEM-1 in E. coli ATCC 25922 (lane 2), ATCC 35218 (lanes 3 to 5), and 907355 (lanes 6 to 9) by Western immunoblotting. Strains were cultured in medium containing no antibiotic (lanes 2, 3, and 6), 4 µg/ml tazobactam (lanes 4 and 7), 4 µg/ml piperacillin (lanes 5 and 8), or 4/4 µg/ml TZP (lane 9) prior to protein sample collection. Molecular mass standards are shown in lane 1 (kilodaltons are indicated to the left of the blot). The estimated molecular mass of Bla TEM-1 is 31.5 kDa. This experiment was repeated on independently collected samples and yielded similar results. (B) Determination of Bla TEM-1 activity in E. coli 907355 via a nitrocefin hydrolysis assay after growth in the presence or absence of 4/4 µg/ml TZP. ATCC 35218 Bla TEM-1 activity is included as a comparison but was not determined (ND) in the presence of TZP due to lack of growth. Units on the y axis denote the micromoles of nitrocefin hydrolyzed per milligram of total protein per minute. The values above each bar on the graph represent the means of 3 experiments, and the error bars indicate the standard deviations of the means.

    Techniques Used: Activity Assay, Western Blot, Cell Culture, Hydrolysis Assay, Comparison

    Estimation of bla TEM-1 copy number by qPCR. bla TEM-1 copy number per cell was measured using a real-time SYBR green qPCR assay after bacterial growth in the absence or presence of 4/4 µg/ml TZP. The copy number was not determined (ND) for ATCC 35218 in the presence of TZP due to lack of growth. The values above each bar on the graph represent the mean results of 3 experiments, and the error bars indicate the standard deviations of the means.
    Figure Legend Snippet: Estimation of bla TEM-1 copy number by qPCR. bla TEM-1 copy number per cell was measured using a real-time SYBR green qPCR assay after bacterial growth in the absence or presence of 4/4 µg/ml TZP. The copy number was not determined (ND) for ATCC 35218 in the presence of TZP due to lack of growth. The values above each bar on the graph represent the mean results of 3 experiments, and the error bars indicate the standard deviations of the means.

    Techniques Used: SYBR Green Assay



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    35218 bla tem 1 genes  (ATCC)
    99
    ATCC 35218 bla tem 1 genes
    Effect of increasing tazobactam concentrations on E. coli susceptibility to TZP. MICs were determined for 907355 (A) and ATCC <t>35218</t> (B) via BMD in the presence of piperacillin (PIP) alone or TZP formulations containing different fixed concentrations (2 to 64 µg/ml) of tazobactam (TAZ). The MIC values in the legend are based on the concentration of piperacillin in the TZP formulation that inhibited growth. The range of MIC values obtained from six experiments is represented within each stacked bar on the graphs.
    35218 Bla Tem 1 Genes, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/35218+bla+tem+1+genes/pmc05915731-86-11-10?v=ATCC
    Average 99 stars, based on 1 article reviews
    35218 bla tem 1 genes - by Bioz Stars, 2026-07
    99/100 stars
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    Effect of increasing tazobactam concentrations on E. coli susceptibility to TZP. MICs were determined for 907355 (A) and ATCC 35218 (B) via BMD in the presence of piperacillin (PIP) alone or TZP formulations containing different fixed concentrations (2 to 64 µg/ml) of tazobactam (TAZ). The MIC values in the legend are based on the concentration of piperacillin in the TZP formulation that inhibited growth. The range of MIC values obtained from six experiments is represented within each stacked bar on the graphs.

    Journal: mBio

    Article Title: Extensive Gene Amplification as a Mechanism for Piperacillin-Tazobactam Resistance in Escherichia coli

    doi: 10.1128/mBio.00583-18

    Figure Lengend Snippet: Effect of increasing tazobactam concentrations on E. coli susceptibility to TZP. MICs were determined for 907355 (A) and ATCC 35218 (B) via BMD in the presence of piperacillin (PIP) alone or TZP formulations containing different fixed concentrations (2 to 64 µg/ml) of tazobactam (TAZ). The MIC values in the legend are based on the concentration of piperacillin in the TZP formulation that inhibited growth. The range of MIC values obtained from six experiments is represented within each stacked bar on the graphs.

    Article Snippet: The copy numbers per cell for E. coli 907355 and ATCC 35218 bla TEM-1 genes were determined using a SYBR green quantitative PCR (qPCR) assay after bacterial growth in cation-adjusted Mueller-Hinton broth (CAMHB) containing or lacking subinhibitory concentrations of TZP (4/4 μg/ml).

    Techniques: Concentration Assay, Formulation

    Effects of TZP on E. coli 907355 Bla TEM-1 levels and β-lactamase enzyme activity. (A) Detection of Bla TEM-1 in E. coli ATCC 25922 (lane 2), ATCC 35218 (lanes 3 to 5), and 907355 (lanes 6 to 9) by Western immunoblotting. Strains were cultured in medium containing no antibiotic (lanes 2, 3, and 6), 4 µg/ml tazobactam (lanes 4 and 7), 4 µg/ml piperacillin (lanes 5 and 8), or 4/4 µg/ml TZP (lane 9) prior to protein sample collection. Molecular mass standards are shown in lane 1 (kilodaltons are indicated to the left of the blot). The estimated molecular mass of Bla TEM-1 is 31.5 kDa. This experiment was repeated on independently collected samples and yielded similar results. (B) Determination of Bla TEM-1 activity in E. coli 907355 via a nitrocefin hydrolysis assay after growth in the presence or absence of 4/4 µg/ml TZP. ATCC 35218 Bla TEM-1 activity is included as a comparison but was not determined (ND) in the presence of TZP due to lack of growth. Units on the y axis denote the micromoles of nitrocefin hydrolyzed per milligram of total protein per minute. The values above each bar on the graph represent the means of 3 experiments, and the error bars indicate the standard deviations of the means.

    Journal: mBio

    Article Title: Extensive Gene Amplification as a Mechanism for Piperacillin-Tazobactam Resistance in Escherichia coli

    doi: 10.1128/mBio.00583-18

    Figure Lengend Snippet: Effects of TZP on E. coli 907355 Bla TEM-1 levels and β-lactamase enzyme activity. (A) Detection of Bla TEM-1 in E. coli ATCC 25922 (lane 2), ATCC 35218 (lanes 3 to 5), and 907355 (lanes 6 to 9) by Western immunoblotting. Strains were cultured in medium containing no antibiotic (lanes 2, 3, and 6), 4 µg/ml tazobactam (lanes 4 and 7), 4 µg/ml piperacillin (lanes 5 and 8), or 4/4 µg/ml TZP (lane 9) prior to protein sample collection. Molecular mass standards are shown in lane 1 (kilodaltons are indicated to the left of the blot). The estimated molecular mass of Bla TEM-1 is 31.5 kDa. This experiment was repeated on independently collected samples and yielded similar results. (B) Determination of Bla TEM-1 activity in E. coli 907355 via a nitrocefin hydrolysis assay after growth in the presence or absence of 4/4 µg/ml TZP. ATCC 35218 Bla TEM-1 activity is included as a comparison but was not determined (ND) in the presence of TZP due to lack of growth. Units on the y axis denote the micromoles of nitrocefin hydrolyzed per milligram of total protein per minute. The values above each bar on the graph represent the means of 3 experiments, and the error bars indicate the standard deviations of the means.

    Article Snippet: The copy numbers per cell for E. coli 907355 and ATCC 35218 bla TEM-1 genes were determined using a SYBR green quantitative PCR (qPCR) assay after bacterial growth in cation-adjusted Mueller-Hinton broth (CAMHB) containing or lacking subinhibitory concentrations of TZP (4/4 μg/ml).

    Techniques: Activity Assay, Western Blot, Cell Culture, Hydrolysis Assay, Comparison

    Estimation of bla TEM-1 copy number by qPCR. bla TEM-1 copy number per cell was measured using a real-time SYBR green qPCR assay after bacterial growth in the absence or presence of 4/4 µg/ml TZP. The copy number was not determined (ND) for ATCC 35218 in the presence of TZP due to lack of growth. The values above each bar on the graph represent the mean results of 3 experiments, and the error bars indicate the standard deviations of the means.

    Journal: mBio

    Article Title: Extensive Gene Amplification as a Mechanism for Piperacillin-Tazobactam Resistance in Escherichia coli

    doi: 10.1128/mBio.00583-18

    Figure Lengend Snippet: Estimation of bla TEM-1 copy number by qPCR. bla TEM-1 copy number per cell was measured using a real-time SYBR green qPCR assay after bacterial growth in the absence or presence of 4/4 µg/ml TZP. The copy number was not determined (ND) for ATCC 35218 in the presence of TZP due to lack of growth. The values above each bar on the graph represent the mean results of 3 experiments, and the error bars indicate the standard deviations of the means.

    Article Snippet: The copy numbers per cell for E. coli 907355 and ATCC 35218 bla TEM-1 genes were determined using a SYBR green quantitative PCR (qPCR) assay after bacterial growth in cation-adjusted Mueller-Hinton broth (CAMHB) containing or lacking subinhibitory concentrations of TZP (4/4 μg/ml).

    Techniques: SYBR Green Assay